Sorry, I didn’t know we had a HW for this week and also didn’t follow about where to find HW. I’ll try not to have the same issue next time, on it for now.

SynBio Read/Write/Edit Homework Assignment

<aside> ⚠️ About your Documentation Make sure to document every step of the in-silico and lab experiments. Make sketches, screenshots, notes, drawings - anything that helps you - and others understand the experiment. Your documentation should help you - and others - to understand the topic. Don’t be afraid to add things that don’t work. Show your failures - and how you did overcome them. Your Documentation should be a description of the amazing journey you are on!

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Part 0: Basics of Gel Electrophoresis

• [x] Watch all lecture and recitation videos.
• [ ] https://mit.zoom.us/rec/play/8G5JKxUGQhk0-TQm68vuJMAZsDbM7gxnBnVhfa1EkqYJvZiye0rwC2u_ykyyF-Ua1DN8JhdyJHOi6e-C.mBawL7GQ5rZ89jLu?canPlayFromShare=true&from=share_recording_detail&continueMode=true&componentName=rec-play&originRequestUrl=https%3A%2F%2Fmit.zoom.us%2Frec%2Fshare%2FdfLSSAjJdq3oVUK9i11nj-q_B9zMa-qvZKLJPYN4IDdq9FpjVi6kwrx7fRj6F5XH.i2UWTathPvKIvbZz
  • [ ] Optionally watch bootcamp

Part 1: Benchling & In-silico Gel Art

See the Gel Art: Restriction Digests and Gel Electrophoresis protocol for details. Overview:

• [x] Make a free account at benchling.com
• [x] Import the Lambda DNA.
• [x] Simulate Restriction Enzyme Digestion with the following Enzymes: EcoRI HindIII BamHI KpnI EcoRV SacI SalI
• [x] Create a pattern/image in the style of Paul Vanouse’s Latent Figure Protocol artworks.

Part 2: Gel Art - Restriction Digests and Gel Electrophoresis

<aside> ⚠️ Optional for Committed Listeners with Lab Access. Mandatory for MIT/Harvard Students. Documentation due start of class Feb 18

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Perform the lab experiment you designed in Part 1 and outlined in the Gel Art: Restriction Digests and Gel Electrophoresis protocol.

Part 3: DNA Design Challenge

<aside> ⚠️ Mandatory for MIT/Harvard Students and Committed Listeners. Due start of class Feb 18

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3.1.     Choose your protein.